重組小鼠堿性FGF(成纖維生長因子)
中文名稱：重組小鼠堿性FGF(成纖維生長因子)英文名稱：Recombinant Mouse Fibroblast growth factor 2/Fibroblast Growth Factor Basic產(chǎn)品規(guī)格：10μg|50μg|500μg|1mg
大腸桿菌表達系統(tǒng)制備，目的基因編碼的Met1-Ser154表達純化而來。
FGF Basic質(zhì)量控制：＞95%(還原性SDS-PAGE)。
FGF Basic制劑：凍干品
FGF Basic保存：
凍干蛋白可在-20℃長期保存，室溫也可穩(wěn)定保存3周。
復(fù)溶蛋白溶液可在4～7℃保存2～7天。
復(fù)溶后建議分裝凍存在-20℃，可保存3個月，應(yīng)避免反復(fù)凍融。
FGF Basic復(fù)溶：
1、試劑開蓋前請先離心。
2、復(fù)溶濃度應(yīng)大于100 μg/ml.
3、凍干蛋白溶于ddH2O中
4、復(fù)溶后根據(jù)使用量分裝保存，避免反復(fù)凍融。
關(guān)于FGF Basic：
FGF basic is one of 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine, and sheep FGF basic. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity. Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis is reminiscent of a variety of pathological conditions |
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